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Gibco™ Advanced MEM

Advanced MEM (Minimum Essential medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.

Overview
15.26 GBP valid until 2025-06-21
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Code: 50

Additional Details:
Additional Details: Weight: 0.59000kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 11530436

Quantity Price
1 £ 22.9 / 500mL
In stock, ready to ship: 711
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For Research Use Only. All usage must comply with product instructions.
 

  • Description and Specification
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Description and Specification

Specification

Cell Line HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Classification Animal Origin
Concentration 1 X
Form Liquid
Product Type MEM (Minimum Essential Medium)
Serum Level Reduced Serum
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
With Additives Low Glucose,Phenol Red,Sodium Pyruvate,Non Essential Amino Acids (NEAA)
With Additives Low Glucose,Phenol Red,Sodium Pyruvate,Non Essential Amino Acids (NEAA)
With Additives Low Glucose,Phenol Red,Sodium Pyruvate,Non Essential Amino Acids (NEAA)
With Additives Low Glucose,Phenol Red,Sodium Pyruvate,Non Essential Amino Acids (NEAA)
Without Additives No Glutamine,No HEPES
Without Additives No Glutamine,No HEPES
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Product Line Gibco™
Quantity 500 mL
Shipping Condition Ambient
Content And Storage Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Advanced MEM (Minimum Essential medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic MEM, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology.i Cells successfully cultured in Advanced DMEM / F-12, with no adaptation, include PK-15, MDCK, Vero, WI-38 and Hep-2.

This Advanced MEM is manufactured as follows:

With: Glucose, Non-essential Amino Acids, Sodium Pyruvate, Phenol Red

Without: L-glutamine, HEPES

The complete formulation is available.

Gibco™ Advanced MEM is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride.

Product Use

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

Advanced MEM requires supplementation with 1-5% Fetal Bovine Serum and 4mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

iPaul Price, Brad Stiles, David Staines, Ethel Evege, Femi Fatunmbi, Kate Wagner, Lori Nestler, David Jayme. Advanced Media as Alternatives to Classical Basal Media. FOCUS, 2003. 25.2:3-6.

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