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IBA Lifesciences BioLock

BioLock is a Biotin blocking solution for lysates and culture supernatants

Additional Details:
Additional Details: Weight: 0.08100kg



Disclaimers: For Research Use Only.

Product Code. 15643608

Quantity Price
1 240.0  € / 50mL
In stock, ready to ship: 6
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Description and Specification

Specification

Capacity (Metric) 50 mL
Type Blocking Solution

BioLock is a ready-to-use biotin blocking solution which contains the tetrameric biotin binding protein avidin. BioLock prevents biotin and biotinylated proteins from binding to Strep-Tactin™ or Strep-Tactin™XT, whereas binding of the Twin-Strep-tag™ and Strep-tag™II is not influenced.

Biotin and/or biotinylated proteins can possibly reduce the binding capacity of Strep-Tactin™ as well as Strep-Tactin™XT and are present in cell lysates and cell culture media, especially from mammalian or insect cells. Thus, if Twin-Strep-tag™ and Strep-tag™II fusion proteins from biotin-containing extracts or media are intended to be purified via Strep-Tactin™ or Strep-Tactin™XT affinity chromatography, biotin has to be masked by addition of BioLock prior to application onto the resin.