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Gibco™ Essential 8™ Medium

Essential 8™ Medium

Overview
294.21 EUR valid until 2025-12-31
Use promo code "24342" to get your promotional price.

Additional Details:
Additional Details: Weight: 0.50000kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 15190617

Quantity Price
1 354.0  € / 500mL
In stock, ready to ship: 219
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Description and Specification

Specification

Cell Line Embryonic Stem Cells (ESCs), Induced Pluripotent Stem Cells (iPSCs)
Cell Type Embryonic Stem Cells (ESCs), Induced Pluripotent Stem Cells (iPSCs)
Classification Xeno-free
Concentration Essential 8 Basal Medium (1X), Essential 8 Supplement (50X)
Form Liquid
Product Type Stem Cell Media
Serum Level Serum-free
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
With Additives Phenol Red
Manufacturing Quality cGMP for medical devices, 21 CFR Part 820 and ISO 13485
Product Line Essential 8™
Quantity 500 mL
Shipping Condition Supplement - dry ice, Basal - ambient
Species Human
Content And Storage • 500 mL basal medium (store at 2—8°C and protect from light)
• 10 mL supplement (store at -5 to -20°C and protect from light)
Culture Type Adherent Cell Culture,Feeder-free Stem Cell Culture (Human, iPS - Induced Pluripotent Stem, Embryonic),Stem Cell (Human, iPS - Induced Pluripotent Stem, Embryonic)
Culture Type Adherent Cell Culture,Feeder-free Stem Cell Culture (Human, iPS - Induced Pluripotent Stem, Embryonic),Stem Cell (Human, iPS - Induced Pluripotent Stem, Embryonic)
Culture Type Adherent Cell Culture,Feeder-free Stem Cell Culture (Human, iPS - Induced Pluripotent Stem, Embryonic),Stem Cell (Human, iPS - Induced Pluripotent Stem, Embryonic)

Essential 8™ Medium is a xeno-free and feeder-free medium specially formulated for the growth and expansion of human pluripotent stem cells (PSCs). Originally developed by Guokai Chen et al. in the laboratory of James Thomson (published as 'E8') and validated by Cellular Dynamics International, Essential 8™ Medium has been extensively tested and proved to maintain pluripotency in multiple iPSC lines. In addition, Essential 8™ Medium has been used to scale up production of iPSCs and shown to support iPSC growth for >50 passages without any signs of karyotypic abnormalities, along with maintaining the ability of iPSCs to differentiate into all three germ line lineages.

Features of Essential 8™ Medium include:

  • Consistent—reduced variability compared to existing feeder-free culture media
  • Robust—reliable and robust cultures with a xeno-free, cGMP, 8-component medium
  • Cost effective—economical and scalable PSC culture compared to other feeder-free media

    Reduced Variability
    Essential 8™ Medium is xeno-free and contains only the 8 essential components needed for stem cell culture. Unlike other media that contain over 20 highly variable ingredients, Essential 8™ Medium is produced under cGMP and has an optimized formulation and growth factor levels to help ensure maximum cell health, pluripotency, and growth, with minimal variability.

    Reliable and Robust Cultures
    Essential 8™ Medium has been show to support pluripotent stem cell growth and provide cultures with superior morphology and growth kinetics compared to other feeder systems.

    Cost Effective
    Essential 8™ Medium is provided in a convenient two-component kit (500 ml basal medium & 10 ml supplement), and when used with vitronectin (VTN-N), provides a cost effective, defined system for feeder-free culture of human pluripotent stem cells (PSCs).

    Essential 8™ Medium is commercialized in partnership with Cellular Dynamics International.

    • Safety and Handling

    [3,4-Dihydroxy-3-cyclobutene-1,2-dione]

    [Warning]

    [{code=EYE DAM. 2, sequence=1, description=Serious eye damage/eye irritation Category 2}, {code=SKIN IRRIT. 2, sequence=2, description=Skin corrosion/irritation Category 2}, {code=STOT SE 3, sequence=3, description=Specific target organ toxicity Category 3}]

    [{code=H315, sequence=1, description=Causes skin irritation.}, {code=H319, sequence=2, description=Causes serious eye irritation.}, {code=H335, sequence=3, description=May cause respiratory irritation.}]

    [{code=P261, sequence=51, description=Avoid breathing dust/fume/gas/mist/vapours/spray.}, {code=P280, sequence=52, description=Wear protective gloves/protective clothing/eye protection/face protection.}, {code=P302+P352, sequence=53, description=IF ON SKIN: Wash with plenty of water/soap.}, {code=P305+P351+P338, sequence=54, description=IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.}]

    [{code=MIXTURE LIST, sequence=1, description=Contains : 3,4-Dihydroxy-3-cyclobutene-1,2-dione}]

    [{code=GHS07, sequence=1, description=EXCLAMATION MARK}]


    Product Identifier
    • 3,4-Dihydroxy-3-cyclobutene-1,2-dione

    Signal Word
    • Warning

    Hazard Category
    • Serious eye damage/eye irritation Category 2
    • Skin corrosion/irritation Category 2
    • Specific target organ toxicity Category 3

    Hazard Statement
    • H315- Causes skin irritation.
    • H319- Causes serious eye irritation.
    • H335- May cause respiratory irritation.

    Precautionary Statement
    • P261- Avoid breathing dust/fume/gas/mist/vapours/spray.
    • P280- Wear protective gloves/protective clothing/eye protection/face protection.
    • P302+P352- IF ON SKIN: Wash with plenty of water/soap.
    • P305+P351+P338- IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.

    Supplemental Information
    • MIXTURE LIST- Contains : 3,4-Dihydroxy-3-cyclobutene-1,2-dione
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