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Gibco™ ExpiCHO™ Expression System Kit

The ExpiCHO Expression System Kit combines the power of rapid, ultra-high-yield transient protein production in suspension culture with the benefit of expression in Chinese hamster ovary (CHO) cells.

Overview
3352.50 EUR valid until 2025-04-18
Use promo code "24107" to get your promotional price.

Additional Details:
Additional Details: Weight: 1.44100kg



Includes: 2 vials frozen ExpiCHO-S™ cells, 1 bottle 1000mL ExpiCHO™ Expression Medium, 1 ExpiFectamine™ CHO transfection kit for 1 L of culture, 1 vial Antibody Expressing Positive Control Vector, 1 bottle 100mL OptiPRO™ SFM; store all components at +2° - +8° C, except frozen cells stored in liquid nitrogen

Product Code. 15441923

Quantity Price
1 3725.0  € / 1 set
In stock, ready to ship: 2
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Description and Specification

Specification

Type Expression System
Protein Tag Untagged
Delivery Type Transfection
Quantity 1 kit
Content And Storage • 2 vials frozen ExpiCHO-S cells
• 1000 mL ExpiCHO Expression Medium
• 1 ExpiFectamine CHO Transfection Kit for 1 L of culture
• 1 vial Antibody Expressing Positive Control Vector
• 100 mL OptiPRO SFM

Store all components at 2-8°C, except frozen cells which should be stored in liquid nitrogen.
Shipping Condition Approved for shipment at Room Temperature or on Wet or Dry Ice
Cell Line CHO-S, SFM Adapted
Expression System Mammalian
Product Line ExpiCHO™
For Use With (Application) Protein Production, Constitutive Expression
Promoter CMV

The ExpiCHO Expression System enables:

  • Milligram to multigram yields of recombinant protein per liter of culture in 5–14 days post-transfection
  • Robust and reproducible transfection results, for greater confidence in results and ability to use CHO cells from research to discovery
  • Transfection efficiency of > 90% for high-density suspension CHO cell culture, with matching transfection enhancer and culture feed to boost transfection performance and protein expression
  • Use of one-third to one-half less plasmid DNA than other transfection reagents 
  • Flexible, reliable culture and transfection protocols with multiple options that readily fit into current transient expression workflows
  • Scalable expression for culture formats from  <1 mL in deep well plates up to 5 L when using Nalgene angled-bottom shake flasks

The components included in the ExpiCHO Expression System Kit are: 2 vials of frozen ExpiCHO-S cells, 1 L of ExpiCHO Expression Medium, 1 ExpiFectamine CHO Transfection Kit sufficient to transfect 1 L of culture, OptiPRO SFM, and an antibody-expressing positive control vector.

ExpiCHO-S cells, ExpiCHO Expression Medium, ExpiFectamine CHO Transfection Kit, and OptiPRO SFM may also be purchased separately.

All components of the system are animal origin–free.

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