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Thermo Scientific™ BpiI (BbsI) (10 U/μL)

Cut GAAGAC(2/6)^ sites with BpiI (BbsI) restriction enzyme, which performs best at 37°C in G buffer (Isoschizomers: BbsI, BpuAI, BstV2I).

Overview
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Code: 88

Additional Details:
Additional Details: Weight: 1.51950kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 10774491

Quantity Price
1 56.0  € / 200 units
In stock, ready to ship: 16
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  • Description and Specification
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Description and Specification

Specification

Concentration 10 U/μL
Methylation Sensitivity Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Methylation Sensitivity Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Methylation Sensitivity Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Enzyme BpiI (BbsI)
Compatible Buffer 10X Buffer G
Sensitive to Heat Inactivation Yes
Optimal Reaction Temperature 37°C
Type IIS RE Yes
Quantity 200 units
Product Type Restriction Enzyme
Research Category Scarless Cloning

5'...G A A G A C (N)2▵...3'

3'...C T T C T G (N)6▵...5'

Conditions for 100% Activity:

  • 1X Buffer G
  • 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 50mM NaCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • BpiI is supplied in:
  • 10mM Tris-HCl (pH7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with BpiI, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: never overlaps — no effect
  • Dcm: never overlaps — no effect
  • CpG: may overlap — no effect
  • EcoKI: never overlaps — no effect
  • EcoBI: may overlap — effect not determined

Digestion of Agarose-embedded DNA:

  • Minimum 5 units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours

Note:

BpiI cleaves downstream of its recognition site and can generate any desired 4 base 5ft.-overhangs. This feature is useful for direct PCR product cloning.

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