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Thermo Scientific™ FastDigest EcoRI

The FastDigest EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer.

Overview
114.48 EUR valid until 2025-04-18
Use promo code "24107" to get your promotional price.

Code: A0

Additional Details:
Additional Details: Weight: 0.45360kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 11883833

Quantity Price
1 159.0  € / 2500 reactions
In stock, ready to ship: 6
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  • Description and Specification
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Description and Specification

Specification

Content And Storage • FastDigest EcoRI (2,500 μL)
• 10X FastDigest Buffer (5 x 1.0 mL)
• 10X FastDigest Green Buffer (5 x 1.0 mL)

Store at –20°C.
Methylation Sensitivity CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Methylation Sensitivity CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Methylation Sensitivity CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Enzyme EcoR I
Compatible Buffer 10X FastDigest Buffer/FastDigest Green Buffer
Sensitive to Heat Inactivation Yes
Optimal Reaction Temperature 37°C
Type IIS RE No
Quantity 5 x 500 μL (2,500 Reactions)
Product Type Restriction Enzyme
Research Category Traditional Cloning
Product Line FastDigest™
Overhang 5' protruding ends
Inactivation Temperature 80°C

Thermo Scientific FastDigest EcoRI restriction enzyme recognizes G^AATTC site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer.

Thermo Scientific FastDigest EcoRI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such as Klenow Fragment (Cat. No. EP0051), T4 DNA Ligase (Cat. No. EL0014), alkaline phosphatases and T4 DNA Polymerase (Cat. No. EP0061) all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.

For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.

Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.

Features

• 100% activity of all FastDigest enzymes in the universal buffer
• 100% buffer compatibility with downstream applications
• Complete digestion in 5–15 minutes
• Direct loading on gels
• No star activity
• 176 FastDigest enzymes available

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern Blot
• Restriction fragment length polymorphism (RFLP)

Note: The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended.

For methylation sensitivity, refer to product specifications.

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