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Gibco™ Advanced DMEM

Advanced DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.

38.06 € - 355.0 €

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Specification

Content And Storage Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Cell Line MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero
Classification Animal Origin
Concentration 1 X
Form Liquid
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Disclaimers: For Research Use Only. Not for use in diagnostic procedures.


Specification

Content And Storage Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Cell Line MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero
Classification Animal Origin
Concentration 1 X
Form Liquid
Product Type DMEM (Dulbecco's Modified Eagle Medium)
Serum Level Reduced Serum
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
With Additives High Glucose,Phenol Red,Sodium Pyruvate,Non Essential Amino Acids (NEAA)
Without Additives No Glutamine,No HEPES
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Product Line Gibco™
Shipping Condition Room Temperature

This Advanced DMEM is manufactured as follows:

With: High Glucose, Non-essential Amino Acids, Sodium Pyruvate, Phenol Red

Without: L-glutamine

The complete formulation is available.

Using Advanced DMEM

Advanced DMEM is unique from other media due to the addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM requires supplementation with 1–5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Order Info Shipping Condition: Room Temperature

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Quantity
11510436
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Gibco™
12491015
 500 mL 38.06 €
500mL
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11520436
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Gibco™
12491023
 10 x 500 mL 355.0 €
Pack of 10
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