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  6. IBA Lifesciences Gravity flow Strep-Tactin™ 4Flow™ high capacity column

IBA Lifesciences Gravity flow Strep-Tactin™ 4Flow™ high capacity column

Product Code. 30117604 Shop All IBA Lifesciences Products
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Capacity (Metric):
1 mL
5 mL
Unit Size:
1mL
5mL
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Product Code. 30117604

Brand: IBA Lifesciences 21253001

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This item is not returnable. View return policy

Description

Strep-Tactin™ 4Flow™ high capacity gravity flow column to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

  • Strep-Tactin™ 4Flow™ high capacity columns are designed for purifying Strep-tag™II and Twin-Strep-tag™ proteins via affinity chromatography in gravity flow
  • Recommended for all protein classes due to the broad pH compatibility, excellent pressure stability, outstanding target purity (>95%), and high binding affinity
  • 4% crosslinked agarose beads coupled to Strep-Tactin™ for straightforward purification of proteins with Strep-tag™II or a Twin-Strep-tag™
  • Targeted elution: no tedious titration steps required and multiple regeneration cycles possible using 100 mM NaOH
  • Ideal alternative for proteins that are difficult to elute from Strep-Tactin™XT 4Flow™ resins
  • Biotinylated proteins also bind to Strep-Tactin™ 4Flow™ high capacity and need to be blocked using BioLock

    • Specifications

    Binding Capacity 20 mg/mL resin
    Capacity (Metric) 5 mL
    Description 4% agarose, crosslinked
    Particle Size 50 to 150 μm
    Form Resin Slurry
    Quantity 5 mL
    Protein Form Tetrameric protein
    Target Proteins tagged with Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin
    Type Column
    Final Product Type Affinity chromatography equipment
    Ligand Type Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin
    Stationary Phase Agarose
    Format Gravity flow column
    Column Type Pre-packed gravity flow column
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