Cytiva HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

65.0 € - 842.0 €

Specification

Description HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Flow Rate 4 mL/min. (1 mL), 5 mL/min. (5 mL)
Material Polypropylene
pH Range 3 to 12
Support Type 6% Highly Cross-linked Agarose
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Specification

Description HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Flow Rate 4 mL/min. (1 mL), 5 mL/min. (5 mL)
Material Polypropylene
pH Range 3 to 12
Support Type 6% Highly Cross-linked Agarose
Content And Storage 4°C to 30°C
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
Type Pre-packed Column

  • Prepacked with Ni Sepharose™ High Performance medium
  • Negligible nickel ion leakage
  • Pressure Max: 3 bar
  • Column material: polypropylene
  • 1 and 5mL columns
  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
  • Manual operation with syringe, pump or chromatography system

Media
  • Matrix: 6% cross-linked agarose
  • Average particle size: 34μm
  • pH stability working range: 3 to 12

1mL columns

  • Bed Dimensions: 7 x 25mm
  • Flow velocity: 4mL/min.

5mL columns
  • Bed Dimensions: 16 x 25mm
  • Flow velocity: 5mL/min.

Product Code Brand Quantity Diameter (Metric) Inner Price ??product.searchResults.label.quantityAvail??
Quantity
10431065
Cytiva
17-5247-01
5 x 1 mL 7 mm 246.0 €
5mL
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11763209
Cytiva
17-5248-01
5 mL 16 mm 219.0 €
5mL
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11773209
Cytiva
17-5248-02
5 x 5 mL 16 mm 842.0 €
25mL
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15167956
Cytiva
29051021
1 mL 7 mm 65.0 €
1mL
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