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Invitrogen™ PureLink™ Genomic DNA Mini Kit
Description
The PureLink™ Genomic DNA Mini Kit enables high-yield, high-purity, genomic DNA (gDNA) extractions from a wide variety of sample types. Use this single kit for genomic DNA purification from blood, tissues, cells, bacteria, swabs, and blood spots, with a familiar silica-based, microcentrifuge spin-column format. Features of the PureLink™ Genomic DNA Mini Kit include:
• Kit flexibility—one kit works with a variety of sample types and sizes
• Ultraclean gDNA—minimal contamination of DNA product means successful downstream application
• Improved design—optimized spin-column design and buffer formulation for better yield and purity
Multiple sample sources, one kit
The PureLink™ Genomic DNA Mini Kit can be used with many different sample types, each with its own optimized protocol outlined in the manual.
Optimized components and protocols
The PureLink™ Genomic DNA Mini Kit includes parts and protocols that improve upon the easy and familiar spin-column methods currently in use. Columns are newly configured to bind and release DNA more efficiently. Each sample source uses a specialized lysis procedure and an optimized lysis buffer formulated to enhance proteinase K activity and eliminate protein contamination. A chaotropic salt buffer promotes stable binding of DNA to the column resin, and powerful wash buffers remove all traces of protein and salt. DNA is eluted in a low-salt buffer to allow for pH stabilization of the DNA in storage.
Higher yields and purity
With PureLink™ Genomic DNA Kits you can expect high yields of high-purity gDNA (determined by A260/A280 measurements), no matter the sample type, from bacteria to tissues to blood to cultured cells. The enhanced PureLink™ Lysis and Wash Buffers help to consistently deliver purified gDNA samples with absorbance reading ratios of ∼1.9 for both A260/A280 and A260/A230, indicating no detectable contamination from carryover of protein or guanidine.
Order Info
Shipping Condition: Room Temperature
Specifications
Specifications
| Content And Storage | • 50 mL PureLink Genomic Lysis/Binding Buffer; room temperature • 45 mL PureLink Genomic Digestion Buffer; room temperature • 50 mL PureLinkGenomic Wash Buffer 1; room temperature • 37.5 mL PureLink Genomic Wash Buffer 2; room temperature • 50 mL PureLink Genomic Elution Buffer; room temperature • 5 mL RNase A (20 mg/mL); room temperature • 5 mL Proteinase K (20 mg/mL); room temperature, or 4°C for long-term storage • 5 x 50 PureLink Spin Columns with Collection Tubes; room temperature • 5 x 100 PureLink Collection Tubes; room temperature |
| Isolation Technology | Silica Spin Column |
| Scale | Mini |
| Sample Type | Bacteria, Cells, Dried Blood Spots, FFPE (Formalin-fixed paraffin-embedded), Mouse/Rat Tail, Saliva, Buccal Swab, Tissue, Whole Blood, Yeast |
| Elution Volume | 25 to 200 μL |
| Final Product Type | gDNA |
| For Use With (Application) | PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| Quantity | 250 Preps |
| Shipping Condition | Room Temperature |
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Safety and Handling
- PURELINK GENOMIC DNA KIT (250)
- Danger
- Acute toxicity Category 4
- Serious eye damage/eye irritation Category 2
- Respiratory sensitiser Category 1
- Skin corrosion/irritation Category 2
- Skin sensitiser Category 1
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H317-May cause an allergic skin reaction.
- H319-Causes serious eye irritation.
- H332-Harmful if inhaled.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P272-Contaminated work clothing should not be allowed out of the workplace.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P284-¢In case of inadequate ventilation! wear respiratory protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P330-Rinse mouth.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
- P362+P364-Take off contaminated clothing and wash it before reuse.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : Guanidine hydrochloride, Proteinase, tritirachium album serine
For Research Use Only. Not for use in diagnostic procedures.
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