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Invitrogen™ SuperScript™ III Reverse Transcriptase

Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) created by the introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability.

Overview
100.67 EUR valid until 2025-06-21
Use promo code "24112" to get your promotional price.

Additional Details:
Additional Details: Weight: 0.05000kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 10432122

Quantity Price
1 151.0  € / 2ku
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  • Description and Specification
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Description and Specification

Specification

Content And Storage

• SuperScript™ III Reverse Transcriptase, 10 μL (2,000 units at 200 U/μL)
• 5X First-strand buffer, 1 mL
• DTT, 500 μL (100 mM)

Store at –20°C.
Format Stand-alone Enzyme
GC-Rich PCR Performance High
Reaction Speed 30 to 50 min.
Technique Reverse Transcription
Optimal Reaction Temperature 50°C
Quantity 2,000 Units
Reverse Transcriptase SuperScript III
Ribonuclease H Activity Reduced
Shipping Condition Dry Ice
Concentration 200 U/μL
Final Product Type First-Strand cDNA
No. of Reactions 10 Reactions
Reaction Format Separate components
Reagent Type Reverse Transcription
Size (Final Product) Up to 12.3 kb
Starting Material RNA

Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) created by the introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability. SuperScript III RT offers higher cDNA yields, improved cDNA lengths, improved efficiency on GC-rich target RNAs, and overall better performance than wild-type MMLV and MMLV RNase H-minus enzymes.

SuperScript RTs are the most highly trusted and widely used RTs with over 50,000 citations, reviews, and publications to date. The enzyme is purified to near homogeneity from E. coli containing the modified pol gene of Moloney Murine Leukemia Virus.

Features of SuperScript III Reverse Transcriptase include:
• First-strand cDNA may be synthesized at temperatures up to 55°C
• cDNA can be generated from 100 bp to >12 kb
• Increased specificity
• High yields of cDNA and full-length products

Note: The latest member of the SuperScript RT family, SuperScript IV Reverse Transcriptase (Cat. No. 18090010), features enhanced thermostability, processivity, yields, and performance with any RNA samples, including those of suboptimal purity or integrity.

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