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  6. Gibco™ N-2 Supplement (100X)

Gibco™ N-2 Supplement (100X)

Product Code. p-7076112
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Quantity:
5 mL
50 mL
Unit Size:
50mL
5mL
This item is not returnable. View return policy
This item is not returnable. View return policy

Description

N-2 Supplement is a versatile serum-free cell culture supplement that supports the growth and viability of various cell types.

Gibco N-2 Supplement is a chemically defined, serum-free supplement based on Bottenstein's N-1 formulation, containing optimized concentrations of insulin, iron-rich holo-transferrin, progesterone, putrescine, and selenite. N-2 Supplement is recommended for growth and expression of neuroblastomas and neural cells in primary cultures from both the peripheral nervous system (PNS) and the central nervous system (CNS).

N-2 Supplement has been demonstrated in wide-ranging applications, such as the culture of neural stem cells, the culture of the SH-SY5Y cell line (subline of the neuroblastoma cell line SK-N-SH), and culture of the HT22 mouse hippocampal neuronal cell line. Additionally, N-2 Supplement adds support to cell culture media used to differentiate pluripotent stem cells (PSCs) to a variety of cell types, including neural and pancreatic-like cell types.

N-2 Supplement may be used with Neurobasal media supplemented with growth factors such as bFGF and EGF. When combined with Dulbecco's Modified Eagle Medium (D-MEM) and fetal bovine serum (FBS), N-2 Supplement also provides suitable supplementation for the culture of human and rodent astrocytes.

  • Each lot is cGMP manufactured
  • Each lot is tested for sterility, endotoxin, and performance
  • Contains insulin, iron-rich holo-transferrin, progesterone, putrescine, and selenite
  • Can be used as a substitute for Bottenstein's N-1 formulation
  • Provided as a 100X concentrate

Choose Gibco N-2 Supplement for a reliable, high-quality product trusted by researchers worldwide.

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Store in freezer (-5 to -20°C) and protect from light.
Cell Type Neural Cells
Classification Serum-free
Concentration 100 X
Culture Type Mammalian Cell Culture
Form Liquid
Product Type Cell Culture Supplement
Serum Level Serum-Free
Sterility Sterile
pH 6 to 8
Quantity 5 mL
Shipping Condition Dry Ice
Tested For Endotoxin, Performance, Sterility
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Safety and Handling

Product Identifier
  • Mercuric acetate
 Supplemental information
  • MIXTURE LIST-Contains : Mercuric acetate

For Research Use Only. Not for use in diagnostic procedures.

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