Macherey-Nagel™ NucleoBond™ Xtra Midi kit for transfection-grade plasmid DNA

Purify transfection-grade plasmid DNA with high yield. The NucleoBond™ Xtra Midi Kit represents the new generation of anion exchanger allowing high yield, ultra-fast.

Overview
Code: LE

Additional Details:
Additional Details: Weight: 2.13210kg Transport: UN number : 1993 Chem class : 3 Pack group : III



Product Code. 12752653

Quantity Price
1 1055.0  € / 100 preps
Estimated Shipment
19-05-2025
Add to basket


  • Description and Specification
  • Documentation
  • Accessories and Spare Parts
  • Safety and Handling
Description and Specification

Specification

Buffer Yes
Format Midi Gravity-flow Column
Isolation Technology Anion Exchange Chromatography Technology
No. of Reactions 100
RNase Yes
Sample Volume <200 mL (high copy), <400 mL (low copy)
Size <300 kbp
Yield 400μg

Features
  • Proven anion-exchange technology – transfection-grade plasmid DNA
  • Patented endotoxin removal by additional washing step – no extra ice incubation necessary
  • Optimized column design – Midi prep in 30 min.
  • Column filter included – high filter flow-rates, parallel lysate clearing and loading onto the column
  • Improved silica material – typical yields of 400μg (Midi)
Applications
  • High-copy plasmids from E. coli
  • Low-copy plasmids from E. coli (increased buffer volumes required)

Documentation Accessories and Spare Parts
Safety and Handling

[Danger]

[{code=FLAM. LIQ. 3, sequence=1, description=Flammable liquid Category 3}, {code=SKIN IRRIT. 2, sequence=2, description=Skin corrosion/irritation Category 2}, {code=EYE DAM. 2, sequence=3, description=Serious eye damage/eye irritation Category 2}, {code=RESP. SENS. 1, sequence=4, description=Respiratory sensitiser Category 1}]

[{code=H226, sequence=1, description=Flammable liquid and vapour.}, {code=H315, sequence=2, description=Causes skin irritation.}, {code=H319, sequence=3, description=Causes serious eye irritation.}, {code=H334, sequence=4, description=May cause allergy or asthma symptoms or breathing difficulties if inhaled.}]

[{code=P210, sequence=51, description=Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.}, {code=P280, sequence=52, description=Wear protective gloves/protective clothing/eye protection/face protection.}, {code=P261, sequence=53, description=Avoid breathing dust/fume/gas/mist/vapours/spray.}, {code=P342+P311, sequence=54, description=If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .}]

[{code=MIXTURE LIST, sequence=1, description=Contains : 2-propanol/sodium hydroxide solution (diluted < 2 %),dodecylsulfate, sodium salt/ethanol (diluted <}]

[{code=GHS02, sequence=1, description=FLAME}, {code=GHS07, sequence=2, description=EXCLAMATION MARK}, {code=GHS08, sequence=3, description=HEALTH HAZARD}]


Signal Word
  • Danger

Hazard Category
  • Flammable liquid Category 3
  • Skin corrosion/irritation Category 2
  • Serious eye damage/eye irritation Category 2
  • Respiratory sensitiser Category 1

Hazard Statement
  • H226- Flammable liquid and vapour.
  • H315- Causes skin irritation.
  • H319- Causes serious eye irritation.
  • H334- May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Precautionary Statement
  • P210- Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P280- Wear protective gloves/protective clothing/eye protection/face protection.
  • P261- Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P342+P311- If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .

Supplemental Information
  • MIXTURE LIST- Contains : 2-propanol/sodium hydroxide solution (diluted < 2 %),dodecylsulfate, sodium salt/ethanol (diluted <