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Macherey-Nagel™ NucleoSpin™ Plasmid DNA

Prepare small scale high-purity plasmid DNA rapidly. NucleoSpin™ Plasmid Columns come with or without a cap lid.

Additional Details:
Additional Details: Weight: 0.53500kg Transport: UN number : 1993 Chem class : 3 Pack group : III



Product Code. 11912252

Quantity Price
1 399.0  € / 250 reactions
Estimated Shipment
03-06-2025
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  • Description and Specification
  • Alternative Products
  • Safety and Handling
Description and Specification

Specification

Format Column
Isolation Technology Silica Membrane Technology
Sequencing Type DNA and RNA Sequencing
For Use With (Application) High-copy plasmids from E. coli
Includes No Lid
No. of Reactions 250 Preps
Product Type Plasmid Columns
Quantity 2 mL
Sample Volume 2 to 10 mL
Size < 50 kbp
Test Time 25min.

Features:

  • For rapid small scale preparation high-purity plasmid DNA
  • Fast procedure – 6 Minipreps in 25 min.
  • High capacity – up to 50μg of plasmid DNA
  • Chaotropic salt wash buffer – extra pure DNA

Applications:

  • High-copy plasmids from E. coli
  • Low-copy plasmids, P1 constructs, or cosmids (increased buffer volumes required)
  • Plasmid DNA from Gram-positive bacteria and plasmid DNA clean-up from reaction mixtures
  • Supplementary protocol for M13 DNA available on request
  • Typical downstream applications: cloning, sequencing, PCR, transformation, restriction analysis

Alternative Products
Safety and Handling

[Danger]

[{code=FLAM. LIQ. 3, sequence=1, description=Flammable liquid Category 3}, {code=ACUTE TOX. 4, sequence=2, description=Acute toxicity Category 4}, {code=SKIN IRRIT. 2, sequence=3, description=Skin corrosion/irritation Category 2}, {code=EYE DAM. 2, sequence=4, description=Serious eye damage/eye irritation Category 2}, {code=RESP. SENS. 1, sequence=5, description=Respiratory sensitiser Category 1}, {code=STOT SE 3, sequence=6, description=Specific target organ toxicity Category 3}]

[{code=H226, sequence=1, description=Flammable liquid and vapour.}, {code=H302, sequence=2, description=Harmful if swallowed.}, {code=H315, sequence=3, description=Causes skin irritation.}, {code=H319, sequence=4, description=Causes serious eye irritation.}, {code=H334, sequence=5, description=May cause allergy or asthma symptoms or breathing difficulties if inhaled.}, {code=H336, sequence=6, description=May cause drowsiness or dizziness.}]

[{code=P261, sequence=51, description=Avoid breathing dust/fume/gas/mist/vapours/spray.}, {code=P342+P311, sequence=52, description=If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .}]

[{code=MIXTURE LIST, sequence=1, description=Contains : sodium hydroxide solution (diluted < 2 %); guanidine hydrochloride; 2-propanol;Rnase}]

[{code=GHS02, sequence=1, description=FLAME}, {code=GHS07, sequence=2, description=EXCLAMATION MARK}, {code=GHS08, sequence=3, description=HEALTH HAZARD}]


Signal Word
  • Danger

Hazard Category
  • Flammable liquid Category 3
  • Acute toxicity Category 4
  • Skin corrosion/irritation Category 2
  • Serious eye damage/eye irritation Category 2
  • Respiratory sensitiser Category 1
  • Specific target organ toxicity Category 3

Hazard Statement
  • H226- Flammable liquid and vapour.
  • H302- Harmful if swallowed.
  • H315- Causes skin irritation.
  • H319- Causes serious eye irritation.
  • H334- May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H336- May cause drowsiness or dizziness.

Precautionary Statement
  • P261- Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P342+P311- If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .

Supplemental Information
  • MIXTURE LIST- Contains : sodium hydroxide solution (diluted < 2 %); guanidine hydrochloride; 2-propanol;Rnase