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Thermo Scientific™ Pierce™ BCA Protein Assay Reagent B

Green-to-blue (A562nm), two-component, precise, detergent-compatible assay reagent to measure total protein concentration vs. protein standard.

Overview
62.83 EUR valid until 2025-06-21
Use promo code "24112" to get your promotional price.

Code: A0

Additional Details:
Additional Details: Weight: 0.05000kg Transport: UN number : 3082 Chem class : 9 Pack group : III



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 10475944

Quantity Price
1 94.25  € / 25mL
In stock, ready to ship: 27
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Description and Specification

Specification

Content And Storage Store at room temperature.
Description Pierce BCA Protein Assay Reagent B
Assay BCA Assay
Detection Method Colorimetric
Product Line Pierce™
Product Type Protein Quantitation Assay
Specificity Not Target-Specific
For Use With (Application) Solution-based Detection, Absorbance
For Use With (Equipment) Spectrophotometer, Microplate Reader
Quantity 25 mL

Used in more labs than any other detergent-compatible protein assay, Pierce BCA Reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity.

Highlights:

  • Colorimetric – estimate visually or measure with a standard spectrophotometer or plate reader (562nm)
  • Excellent uniformity – exhibits less protein-to-protein variation than dye-binding methods
  • Compatible – unaffected by typical concentrations of most ionic and nonionic detergents
  • Moderately fast – much easier and four times faster than the classical Lowry method
  • High linearity – linear working range for BSA equals 20 to 2000μg/mL
  • Sensitive – detect down to 5μg/mL with the enhanced protocol

BCA Protein Assay Applications:

  • Studying protein:protein interactions
  • Measuring column fractions after affinity chromatography
  • Estimating percent recovery of membrane proteins from cell extracts
  • High-throughput screening of fusion proteins

Documentation

Technical documents

Safety and Handling

[Pierce(R) BCA Protein Assay Reagent B, 25 mL]

[Warning]

[{code=AQUATIC ACUTE 1, sequence=1, description=ACUTE AQUATIC Acute 1}, {code=AQUA CHRONIC 2, sequence=2, description=LONG-TERM AQUATIC HAZARD Chronic 2}]

[{code=H400, sequence=1, description=Very toxic to aquatic life.}, {code=H411, sequence=2, description=Toxic to aquatic life with long lasting effects.}]

[{code=P273, sequence=51, description=Avoid release to the environment.}, {code=P391, sequence=52, description=Collect spillage.}]

[{code=MIXTURE LIST, sequence=1, description=Contains : Copper (II) sulfatepentahydrate}]

[{code=GHS09, sequence=1, description=ENVIRONMENT}]


Product Identifier
  • Pierce(R) BCA Protein Assay Reagent B, 25 mL

Signal Word
  • Warning

Hazard Category
  • ACUTE AQUATIC Acute 1
  • LONG-TERM AQUATIC HAZARD Chronic 2

Hazard Statement
  • H400- Very toxic to aquatic life.
  • H411- Toxic to aquatic life with long lasting effects.

Precautionary Statement
  • P273- Avoid release to the environment.
  • P391- Collect spillage.

Supplemental Information
  • MIXTURE LIST- Contains : Copper (II) sulfatepentahydrate
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