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Invitrogen™ Platinum™ Hot Start PCR Master Mixes (2X)

Invitrogen Platinum Hot Start PCR Master Mix offers Platinum Taq DNA polymerase in an optimized PCR buffer with magnesium and dNTPs for convenient PCR setup.

Overview
294.65 EUR valid until 2025-06-30
Use promo code "24074" to get your promotional price.

Additional Details:
Additional Details: Weight: 0.25000kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 15420594

Quantity Price
1 396.0  € / 1 set
In stock, ready to ship: 8
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  • Description and Specification
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Description and Specification

Specification

Content And Storage • Platinum Green Hot Start PCR 2X Master Mix, 4 x 1.25 mL
• Platinum GC Enhancer, 2 x 1.25 mL
• Nuclease-free water, 4 x 1.25 mL

Store at -20°C in a non-frost-free freezer.
Format Tube
GC-Rich PCR Performance Medium
Polymerase Platinum Taq DNA Polymerase
Reaction Speed Standard
Label or Dye Green
Product Type Hot Start PCR Master Mix
Quantity 200 reactions
Shipping Condition Dry Ice
For Use With (Application) Hot-start PCR
Concentration 2X
Fidelity (vs. Taq) 1X
Hot Start Built-In Hot Start
No. of Reactions 200 Reactions
Overhang 3'-A
Reaction Format SuperMix or Master Mix
Size (Final Product) 5 kb or less
Starting Material DNA
Color Green

Platinum Green Hot Start PCR Master Mix additionally contains a density reagent and two tracking dyes for direct loading of PCR products on gels.

Platinum Hot Start PCR Master Mix provides a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs, thus reducing the number of pipetting steps during PCR reaction setup. It is supplied with a separate vial of Platinum GC Enhancer, formulated for specific amplification and improved yields of GC-rich targets.

Platinum Taq DNA Polymerase is a recombinant Taq DNA polymerase complexed with proprietary antibodies that inhibit enzyme activity at room temperature, reducing nonspecific target amplification at lower temperatures. The antibodies dissociate during the initial PCR denaturation step, and DNA polymerase regains its full activity. Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a non-template-dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends and has a 5' to 3' exonuclease activity.

Features

  • High specificity and increased yields with antibody-mediated hot-start PCR
  • Convenient room temperature reaction setup
  • Versatile formulation for broad range of amplicons
  • Direct gel loading with green PCR master mix

Applications

  • Hot Start PCR
  • Genotyping
  • Gene expression profiling
  • Next-generation sequencing

Notes

  • Platinum II Taq Hot-start DNA Polymerase is recommended superior PCR performance.
  • For applications that require PCR product analysis by absorbance or fluorescence excitation, colorless format is recommended.

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