Macherey-Nagel™ Protino Ni-NTA Agarose for His-tag Protein Purification

Method of choice for His-tag protein purification with best performance.

Additional Details:
Additional Details: Weight: 0.50000kg



Product Code. 11952192

Quantity Price
1 4455.0  € / 500mL
Estimated Shipment
21-05-2025
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  • Description and Specification
  • Alternative Products
  • Safety and Handling
Description and Specification

Specification

Binding Capacity <50 mg/mL settled agarose
Description Aqueous suspension
Content And Storage 4°C to 8°C
Quantity 500 mL
For Use With (Application) Purification of His-tag proteins
Target Protein
Type Agarose
Ligand Type NTA (nitrilotriacetic acid)

  • Enable fast and convenient purification of recombinant polyhistidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
  • Proteins from any expression system can be purified under native or denaturing conditions.
  • Binding of protein is based on the interaction between the polyhistidine tag of the recombinant protein and immobilized Ni2+ ions.
  • Consists of the chelating ligand nitrilotriacetic acid (NTA) immobilized on 6% cross-linked agarose beads that are suitable for batch binding, gravity flow, and FPLC™ columns.
  • The resin is precharged with Ni2+ ions and therefore ready to use.
  • Technology: Immobilized metal ion affinity chromatography
  • Batch binding, gravity flow column chromatography, MPLC / FPLC applications
  • Matrix: 6 % beaded agarose (cross-linked), precharged with Ni2+
  • Bead size: 45 to 165 μm

Alternative Products
Safety and Handling

[Warning]

[{code=FLAM. LIQ. 3, sequence=1, description=Flammable liquid Category 3}, {code=SKIN SENS. 1, sequence=2, description=Skin sensitiser Category 1}]

[{code=H226, sequence=1, description=Flammable liquid and vapour.}, {code=H317, sequence=2, description=May cause an allergic skin reaction.}]

[{code=P210, sequence=51, description=Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.}, {code=P261, sequence=52, description=Avoid breathing dust/fume/gas/mist/vapours/spray.}, {code=P280, sequence=53, description=Wear protective gloves/protective clothing/eye protection/face protection.}]

[{code=MIXTURE LIST, sequence=1, description=Contains : ethanol (diluted < 20 %) (denatured with1%IPA/1%MEK, acc.2016/1867/EU), resin suspension with Ni in}]

[{code=GHS02, sequence=1, description=FLAME}, {code=GHS07, sequence=2, description=EXCLAMATION MARK}]


Signal Word
  • Warning

Hazard Category
  • Flammable liquid Category 3
  • Skin sensitiser Category 1

Hazard Statement
  • H226- Flammable liquid and vapour.
  • H317- May cause an allergic skin reaction.

Precautionary Statement
  • P210- Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P261- Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P280- Wear protective gloves/protective clothing/eye protection/face protection.

Supplemental Information
  • MIXTURE LIST- Contains : ethanol (diluted < 20 %) (denatured with1%IPA/1%MEK, acc.2016/1867/EU), resin suspension with Ni in