IBA Lifesciences Strep-Tactin™ Conjugate

Strep-Tactin™ conjugates with AP or HRP for detection of Strep-tag™II and Twin-Strep-tag™ fusion proteins

Overview

Code: LE

Additional Details:

Additional Details: Weight: 0.00100kg

Disclaimers: For Research Use Only.

Product Code. 12698247

Quantity	Price
1	180.0 € / Each

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Estimated Shipment
02-06-2025

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Description and Specification

Content And Storage	4°C
Product Type	HRP Conjugate

The streptavidin variant Strep-Tactin™ is available as conjugate with horseradish peroxidase (HRP) or with alkaline phosphatase (AP) for fast and directed detection of N-terminally, C-terminally, or internally tagged Strep-tag™II or Twin-Strep-tag™ fusion proteins transferred to a blotting membrane or captured on a microplate.
Both conjugates are suitable for chromogenic reaction, whereas Strep-Tactin™ HRP is applicable for chemiluminescence reaction as well.
Depending on the host organism, protein extracts may contain small amounts of biotinylated proteins, e.g., the biotin carboxyl carrier protein (BCCP, 22 kDa) of E. coli.
Biotin Blocking Buffer is recommended for blocking of such biotinylated proteins on western blots prior to detection of Strep-tag™II or Twin-Strep-tag™ fusion proteins with Strep-Tactin™ HRP to prevent the detection of false positive bands.
For this purpose, Biotin Blocking Buffer contains the tetrameric biotin binding protein avidin, which selectively binds biotinylated proteins.

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