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Invitrogen™ SuperScript™ IV Reverse Transcriptase

SuperScript IV Reverse Transcriptase (RT) is a proprietary MMLV mutant with superior robustness and reliability in RT reactions.

Additional Details:
Additional Details: Weight: 0.05000kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 15307696

Quantity Price
1 446.0  € / 10000 units
In stock, ready to ship: 1794
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  • Description and Specification
  • Alternative Products
  • Safety and Handling
Description and Specification

Specification

Content And Storage

• SuperScript IV RT, 50 μL (200 U/μL)
• 5X RT buffer, 1 mL
• 0.1 M DTT, 500 μL

Store at -15°C to –25°C.
Format Stand-alone Enzyme
GC-Rich PCR Performance High
Reaction Speed 10 min.
Technique Reverse Transcription
Optimal Reaction Temperature 50°C
Quantity 10,000 Units
Reverse Transcriptase SuperScript IV
Ribonuclease H Activity Reduced
Shipping Condition Dry Ice
Concentration 200 U/μL
Final Product Type First-Strand cDNA
No. of Reactions 50 Reactions
Reaction Format Separate components
Reagent Type Reverse Transcription
Size (Final Product) Up to 12.3 kb
Starting Material RNA

SuperScript IV Reverse Transcriptase (RT) is a proprietary MMLV mutant with superior robustness and reliability in RT reactions. It is significantly improved over SuperScript III RT in inhibitor resistance, processivity, and reaction speed, while retaining all the benefits of that enzyme, including increased thermostability, highly efficient full-length cDNA synthesis, and reduced RNase activity. SuperScript IV RT is designed to provide reliable, consistent, and fast cDNA synthesis in the presence of inhibitors found in a wide variety of samples that cause other currently available RTs to perform inefficiently.

SuperScript IV RT offers advantages at standard as well as elevated temperatures over cloned AMV and ThermoScript RT, including higher sensitivity, inhibitor tolerance, and ability to work with degraded RNA.

Features of SuperScript IV Reverse Transcriptase include:
• Significantly improved resistance to inhibitors that can interfere with cDNA synthesis
• Robust and specific cDNA synthesis in a wide range of sample types
• Faster reverse transcriptase reaction reduces incubation time from >50 minutes to 10 minutes
• Better processivity when compared to SuperScript III RT

SuperScript IV RT is the top choice for all RT-PCR and qRT-PCR applications in the SuperScript product family when reproducibility and reliability is the primary concern. It is also our top performing RT when inhibitors in the RNA sample may interfere with cDNA synthesis, leading to biases in gene expression studies.

Source
Purified from E. coli expressing the pol gene of M-MLV, modified to increase thermostability and half-life, processivity, inhibitor resistance and to reduce RNase H activity

Unit definition
One unit of SuperScript IV RT is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min at 37°C using poly(A) oligo(dT)12-18 as a template/primer.

Unit reaction conditions
50 mM Tris-HCl (pH 8.3), 4 mM MgCl2, 10 mM DTT, 50 mM KCl, 0.5 mM dTTP, 0.4 MBq/mL [3H]-dTTP, 0.4 mM poly(A) oligo (dT)12-18 and enzyme in 20 μl for 10 min at 37°C.

Alternative Products
Safety and Handling

[SuperScript IV Reverse Transcriptase]

[Warning]

[{code=EYE DAM. 2, sequence=1, description=Serious eye damage/eye irritation Category 2}]

[{code=H319, sequence=1, description=Causes serious eye irritation.}]

[{code=P264, sequence=51, description=Wash hands thoroughly after handling.}, {code=P280, sequence=52, description=Wear protective gloves/protective clothing/eye protection/face protection.}, {code=P305+P351+P338, sequence=53, description=IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.}, {code=P337+P313, sequence=54, description=If eye irritation persists: Get medical advice/attention.}]

[{code=MIXTURE LIST, sequence=1, description=Contains : (R*,R*)-1,4-dimercapto-2,3-Butanediol}]

[{code=GHS07, sequence=1, description=EXCLAMATION MARK}]


Product Identifier
  • SuperScript IV Reverse Transcriptase

Signal Word
  • Warning

Hazard Category
  • Serious eye damage/eye irritation Category 2

Hazard Statement
  • H319- Causes serious eye irritation.

Precautionary Statement
  • P264- Wash hands thoroughly after handling.
  • P280- Wear protective gloves/protective clothing/eye protection/face protection.
  • P305+P351+P338- IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P337+P313- If eye irritation persists: Get medical advice/attention.

Supplemental Information
  • MIXTURE LIST- Contains : (R*,R*)-1,4-dimercapto-2,3-Butanediol