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Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.

Overview
145.35 EUR valid until 2025-05-31
Use promo code "25977" to get your promotional price.

Code: A0

Additional Details:
Additional Details: Weight: 2.72160kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 10391544

Quantity Price
1 157.0  € / 20mL
In stock, ready to ship: 23
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  • Description and Specification
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Description and Specification

Specification

Product Type Substrate
Detection Method Chemiluminescence
Form Liquid
For Use With (Application) Western Blot
Membrane Compatibility Nitrocellulose, PVDF
Product Line SuperSignal™
Quantity 20 mL
Substrate Horseradish Peroxidase
Recommended Antibody Concentrations 2°: 1:100-500K (2-10ng/mL), 1°: 1:5K (10- 200ng/mL)
Sensitivity Low femtogram-level
Signal Duration 6 to 8 hr.
Sufficient For 2 Mini-Gel Blots, 200 sq. cm of Membrane
Kit Contents Stable Peroxide Buffer (10 mL),Luminol/Enhancer (10 mL)
Kit Contents Stable Peroxide Buffer (10 mL),Luminol/Enhancer (10 mL)
Content And Storage Sufficient For: 2 mini-gel blots; 200 cm2 of membrane
• Luminol/Enhancer, 10 mL
• Stable Peroxide Buffer, 10 mL

Store reagents at 4°C.

SuperSignal West Femto Substrate Maximum Sensitivity Substrate is an ultra-sensitive chemiluminescent substrate system for Western blotting with horseradish peroxidase (HRP) enzyme. The substrate kit enables detection of low femtogram (that's mid-zeptomole!) amounts of protein deposited on nitrocellulose or PVDF membrane and probed with appropriate primary and secondary antibodies. When combined with optimized antibody concentrations and blocking buffers, SuperSignal West Femto Substrate enables detection of target proteins in amounts that are small to be seen with typical ECL substrates.

Highlights:

  • ECL substrate – an enhanced chemiluminescent substrate for detection of horseradish peroxidase (HRP) activity from antibodies and other Western blot probes

  • Sensitive – detect low-femtogram (mid-zeptomole) amounts of protein in bands on nitrocellulose or PVDF membranes

  • Quantitative – produces quantitative signal that is measurable over two orders of magnitude

  • Saves on antibody – optimized for very dilute antibody concentrations; dilute primary antibodies 5000- to 100,000-fold and secondary antibodies 100,000 to 500,000-fold (from 1mg/mL stocks)

  • Intense signal – easy to capture an image by exposure to autoradiography film or via a CCD camera or similar imaging system

  • Excellent signal duration – 8 hours of usable light-output when conditions are optimized

  • Stable reagent – kit components are stable for 1 year at 4°C or 6 months at room temperature

Alternative Products
Documentation

Technical documents

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