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Thermo Scientific™ T4 DNA Ligase (5 U/μL)

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.

Overview
228.62 EUR valid until 2025-04-18
Use promo code "24107" to get your promotional price.

Code: A0

Additional Details:
Additional Details: Weight: 0.04990kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 10621441

Quantity Price
1 322.0  € / 5000 units
In stock, ready to ship: 37
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  • Description and Specification
  • Alternative Products
  • Safety and Handling
  • Special Offers
Description and Specification

Specification

Concentration 5 U/μL
Enzyme DNA Ligase
Compatible Buffer 10X T4 DNA Ligase Buffer
Quantity 5 x 1,000 units
Product Type T4 DNA Ligase

Highlights

  • Active in Themo Scientific restriction enzyme, PCR, and RT buffers (when supplemented with ATP)
  • Fast – sticky-end ligation is completed in 10 minutes at room temperature
  • Supplied with PEG solution for efficient blunt-end ligation

Applications

  • Cloning of restriction enzyme generated DNA fragments
  • Cloning of PCR products
  • Joining of double-stranded oligonucleotide linkers or adaptors to DNA
  • Site-directed mutagenesis
  • Amplified fragment length polymorphism (AFLP)
  • Ligase-mediated RNA detection (see Reference 3)
  • Nick repair in duplex DNA, RNA or DNA/RNA hybrids
  • Self-circularization of linear DNA.

Notes

  • Binding of T4 DNA Ligase to DNA may result in a band shift in agarose gels. To avoid this, incubate samples with 6X DNA Loading Dye & SDS Solution at 70°C for 5 min or 65°C for 10 minutes and chill on ice prior to electrophoresis.
  • The volume of the ligation reaction mixture should not exceed 10% of the competent cell volume in the transformation process.
  • Prior to electro-transformation, remove T4 DNA Ligase from the ligation mixture by spin column or chloroform extraction. The extracted DNA can be further precipitated with ethanol.

Alternative Products
Safety and Handling

[T4 DNA Ligase (5 U/µL)]

[Warning]

[{code=EYE DAM. 2, sequence=1, description=Serious eye damage/eye irritation Category 2}]

[{code=H319, sequence=1, description=Causes serious eye irritation.}]

[{code=P264, sequence=51, description=Wash hands thoroughly after handling.}, {code=P280, sequence=52, description=Wear protective gloves/protective clothing/eye protection/face protection.}, {code=P305+P351+P338, sequence=53, description=IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.}, {code=P337+P313, sequence=54, description=If eye irritation persists: Get medical advice/attention.}]

[{code=MIXTURE LIST, sequence=1, description=Contains : (R*,R*)-1,4-dimercapto-2,3-butanediol}]

[{code=GHS07, sequence=1, description=EXCLAMATION MARK}]


Product Identifier
  • T4 DNA Ligase (5 U/µL)

Signal Word
  • Warning

Hazard Category
  • Serious eye damage/eye irritation Category 2

Hazard Statement
  • H319- Causes serious eye irritation.

Precautionary Statement
  • P264- Wash hands thoroughly after handling.
  • P280- Wear protective gloves/protective clothing/eye protection/face protection.
  • P305+P351+P338- IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P337+P313- If eye irritation persists: Get medical advice/attention.

Supplemental Information
  • MIXTURE LIST- Contains : (R*,R*)-1,4-dimercapto-2,3-butanediol
Special Offers