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Cytiva HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Overview
Code: GE

Additional Details:
Additional Details: CAS Number: 7440-02-0 Weight: 0.14400kg



Product Code. 11773209

Quantity Price
1 842.0  € / 25mL
In stock, ready to ship: 15
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  • Description and Specification
  • Alternative Products
  • Safety and Handling
Description and Specification

Specification

Bed Volume 5 mL
Diameter (English) Inner 0.6 in.
Diameter (Metric) Inner 16 mm
Description HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Flow Rate 4 mL/min. (1 mL), 5 mL/min. (5 mL)
Material Polypropylene
pH Range 3 to 12
Support Type 6% Highly Cross-linked Agarose
Content And Storage 4°C to 30°C
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
Quantity 5 x 5 mL
Type Pre-packed Column

  • Prepacked with Ni Sepharose™ High Performance medium
  • Negligible nickel ion leakage
  • Pressure Max: 3 bar
  • Column material: polypropylene
  • 1 and 5mL columns
  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
  • Manual operation with syringe, pump or chromatography system

Media
  • Matrix: 6% cross-linked agarose
  • Average particle size: 34μm
  • pH stability working range: 3 to 12

1mL columns

  • Bed Dimensions: 7 x 25mm
  • Flow velocity: 4mL/min.

5mL columns
  • Bed Dimensions: 16 x 25mm
  • Flow velocity: 5mL/min.

Alternative Products
Safety and Handling

[Warning]

[{code=FLAM. LIQ. 3, sequence=1, description=Flammable liquid Category 3}]

[{code=H226, sequence=1, description=Flammable liquid and vapour.}]

[{code=P280, sequence=51, description=Wear protective gloves/protective clothing/eye protection/face protection.}, {code=P210, sequence=52, description=Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.}, {code=P241, sequence=53, description=Use explosion-proof electrical/ventilating/lighting/ equipment.}, {code=P303+P361+P353, sequence=54, description=IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.}, {code=P235, sequence=55, description=Keep cool.}, {code=P501, sequence=56, description=Dispose of contents/container to .}]

[{code=MIXTURE LIST, sequence=1, description=Contains : Ethanol, Nickel}, {code=UNKNOWN ACUTE, sequence=2, description=16.5 per cent of the mixture consists of component(s) of unknown acute toxicity.}]

[{code=GHS02, sequence=1, description=FLAME}]


Signal Word
  • Warning

Hazard Category
  • Flammable liquid Category 3

Hazard Statement
  • H226- Flammable liquid and vapour.

Precautionary Statement
  • P280- Wear protective gloves/protective clothing/eye protection/face protection.
  • P210- Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241- Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P303+P361+P353- IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P235- Keep cool.
  • P501- Dispose of contents/container to .

Supplemental Information
  • MIXTURE LIST- Contains : Ethanol, Nickel
  • UNKNOWN ACUTE- 16.5 per cent of the mixture consists of component(s) of unknown acute toxicity.